New light “trigger” used for CRISPR gene editing research

Scientists have developed a new way of slicing genetic material by using light combined with CRISPR.

Researchers from Johns Hopkins University used a particular RNA molecule that is light sensitive. They then exposed the molecule to certain light wavelengths. The exposure to the light acted as a “trigger” which allowed the scientists to use the CRISPR method of cutting DNA molecules, according to the National Science Foundation. 

After the molecules were sliced with CRISPR, the researchers observed that proteins within the DNA were able to repair themselves minutes after being triggered. They found that it took only 30 seconds for over half of the sites targeted for DNA to be cut. That allowed the researchers to make observations on how the cells repaired themselves in real-time.

Manju Hingorani, with the National Science Foundation, said, “with this new CRISPR technology, scientists can get to the right place on the genome at the right time to catch the action," according to the National Science Foundation. 

The National Science Foundation helped fund the project. The study was published in the journal Science.